cDNA Cloning and Functional Analysis of Minipig Uridine Diphosphate-Glucuronosyltransferase 1A1
نویسندگان
چکیده
منابع مشابه
Dimerization of human uridine diphosphate glucuronosyltransferase allozymes 1A1 and 1A9 alters their quercetin glucuronidation activities.
Uridine diphosphate glucuronosyltransferase 1A (UGT1A) is a major phase II drug-metabolism enzyme superfamily involved in the glucuronidation of endobiotics and xenobiotics in humans. Many polymorphisms in UGT1A genes are reported to inhibit or decrease UGT1A activity. In this study, two UGT1A1 allozymes, UGT1A1 wild-type and a splice mutant, as well as UGT1A9 wild-type and its three UGT1A9 all...
متن کاملCompartmentation of uridine diphosphate glucuronosyltransferase: effect of uridine diphosphate N-acetylglucosamine on the apparent V of glucuronidation [proceedings].
UDP-glucuronosyltransferase, quantitatively the most important enzyme of phase I1 of mammalian drug metabolism, is strongly bound to hepatic endoplasmic-reticulum membranes and exhibits the phenomenon of latency in fresh microsomal preparations. Latency can be removed and glucuronidation increased by many membrane-lysis procedures and also by UDP-N-acetylglucosamine: this is non-lytic and is a ...
متن کاملAnalysis of uridine diphosphate glucuronosyl transferase 1A1 gene mutations in neonates with unconjugated hyperbilirubinemia.
This study was carried out to analyze uridine diphosphate (UDP)-glucuronosyltransferase 1A1 (UGT1A1) gene mutations in neonates with unconjugated hyperbilirubinemia, from two different ethnic groups. Polymerase chain reaction and gene sequencing were used to analyze the differences in genotypes and allele frequencies of different gene mutations among the ethnic groups; this was followed by chec...
متن کاملEnzymatic Synthesis of Uridine Diphosphate Xylose and Uridine Diphosphate Arabinose.
It has been shown previously' that mung bean seedlings contain a mixture of sugar nucleotides consisting of UDPG,2 UDPGal, UDPXy, and UDPAr. In the present communication evidence is presented that these bean seedlings contain an enzymatic system capable of catalyzing the reversible formation of UDPXy and UDPAr from UTP and a-D-Xy-l-P. When a-D-Xy-l-P and UTP were incubated in the presence of an...
متن کاملDemonstration of functional heterogeneity of hepatic uridine diphosphate glucuronosyltransferase activities after administration of 3-methylcholanthrene and phenobarbital to rats.
After the administration of 3-methylcholanthrene to adult male rats, activities of hepatic UDP-glucuronosyltransferase towards six from a group of 12 substrates were stimulated by 250-350%. Activities towards the remaining six substrates were unaffected. Conversely, after phenobarbital administration, activities formerly stimulated by 3-methylcholanthrene remained unchanged, and the other six a...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2013
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.b12-00986